Bacillus subtilis is an aerobic, endospore-forming Gram-positive rod in the family Bacillaceae, commonly found in soil and associated plant surfaces. It is the best-studied Gram-positive model organism, with strain 168 serving as a reference for sporulation, competence, biofilm formation, and secondary metabolism, and it holds GRAS status for use in industrial enzyme production and as a probiotic in animal feed. B. subtilis has a genome of approximately 4.2 Mb encoding a large number of secondary metabolite biosynthetic clusters, and it displays natural genetic competence, making it a workhorse for Gram-positive genetics and biotechnology.
Derived from 2,364 genomes: 418 from RefSeq and 1,946 from other sources. For the derivation pipeline and the PASS / WARN / FAIL verdict model, see the methods page for qualibact-v1.0.
This table summarises the distribution of each metric, including standard deviation, mean, median, and percentiles.
A combined summary table across all species is available on the summary page.
| Metric | Distribution | n | Mean | SD | Min | Q1 | Median | Q3 | Max |
|---|---|---|---|---|---|---|---|---|---|
| N50 | non-normal | 1,946 | 1,031,987 | 683,319 | 31,505 | 580,312 | 931,703 | 1,048,613 | 2,294,043 |
| no_of_contigs | non-normal | 1,946 | 44.18 | 36.26 | 16 | 26 | 33 | 40 | 348 |
| longest | non-normal | 1,946 | 1,298,540 | 621,807 | 106,497 | 1,015,372 | 1,073,408 | 2,057,004 | 2,294,043 |
| GC_Content | non-normal | 1,946 | 43.51 | 0.21 | 42.93 | 43.29 | 43.44 | 43.72 | 44.03 |
| Completeness_Specific | non-normal | 1,946 | 100 | 0.01 | 99.71 | 100 | 100 | 100 | 100 |
| Contamination | non-normal | 1,946 | 0.22 | 0.28 | 0 | 0.04 | 0.09 | 0.43 | 2.3 |
| Total_Coding_Sequences | non-normal | 1,946 | 4,165 | 156.51 | 3,807 | 4,012 | 4,221 | 4,315 | 4,635 |
| Genome_Size | non-normal | 1,946 | 4,115,334 | 123,702 | 3,829,812 | 3,990,786 | 4,161,774 | 4,245,874 | 4,358,602 |
Full statistics including KS test vs RefSeq and Wasserstein distance are in the downloadable summary.csv.
Derived from 2,364 genomes including 418 RefSeq references
Both Fail and Warn bands shown as the published rounded values — easier to cite and consistent across the species page, CSV downloads, and downstream QC tools.
| Metric | Fail below | Warn below | Warn above | Fail above |
|---|---|---|---|---|
| Genome_Size | - | - | - | - |
| GC_Content | - | - | - | - |
| Total_Coding_Sequences | - | - | - | - |
| Completeness_Specific | - | - | - | - |
| Contamination | - | - | - | - |
| N50 | - | - | - | - |
| no_of_contigs | - | - | - | - |
| longest | - | - | - | - |
How to read this: A value outside a failure limit fails QC. Within the failure limits, values beyond a warning limit are borderline and worth a manual look; values within the warning limits pass that criterion. A dash means that no bound is defined on that side.